Kelman's
TZC Agar (4)
TZC
Stock Solution: Dissolve 1 g of
2,3,5-triphenyl tetrazolium chloride (TZC) in 100 ml of distilled
water. Place in a light-proof capped bottle, and autoclave for
only 8 min or sterilize by filtration. Store refrigerated.
Basal
Medium:
|
Dextrose
|
10 g (or 2.5 g)*
|
|
Peptone
|
10 g
|
|
Casamino acids
|
1 g
|
|
Agar
|
18 g
|
|
Distilled water
|
1000 ml
|
* Modification by reducing to 2.5 g
results in a better growth rate, especially of the potato strain.
Sucrose can be used as a substitute.
Preparation
for plating:
The basal medium can be autoclaved and stored, then melted as
needed. To each liter of cooled, melted basal medium, add 5 ml of
the TZC stock solution to give a final concentration of 0.005%
(aliquots of 200 ml are recommended for ease of handling; to these, 1
ml of TZC stock solution is added).
Plating and storing:
Pour approximately 20 ml per petri plate. When the agar is set,
store inverted. Keep 1-2 days before using to permit surface
drying. Long term storage may result in poor bacterial growth.
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