NC State University

Research areas

Our lab studies the behavioral ecology of insect societies, with a primary focus on the proximate and ultimate mechanisms of honey bee queen behavior and reproduction. In doing so, we attempt to address questions of basic science that have practical relevance. Our philosophy is to integrate a general understanding of bee biology to help improve overall colony health and productivity; in an era when the honey bee population is being severely impacted by any number of factors, we feel that it is incumbent upon the honey bee scientific community to become more proactive in asking questions that address not just basic (long-term) or applied (short-term) questions, but both.

Polyandry and genetic diversity

Queen
Our lab has a protracted history of investigations into the adaptive benefits of intracolony genetic diversity. Specifically, we have investigated the fitness advantages of queen polyandry (mating with multiple males) and its consequences on colony phenotype. We also investigate the regulation of honey bee reproduction by looking at the genomic and physiological changes in queen bees during mating. We have used techniques such as gene-expression, classical behavioral observation, and GC-MS to determine how virgin queens (who are receptive to mating) transition to laying queens (who never mate again in their entire lifetimes). Major collaborators: Tom Seeley, Rob Page, Christina Grozinger

Molecular and pollinator ecology

Forager
Insect pollinators face numerous threats—including pathogens, agrochemical exposure, and reduced habitat—which put food crops at risk. Our collaborative work on pollinator diversity and efficiency in commercial blueberry production has developed interdisciplinary methods that integrate risk assessment into pollination management strategies. Moreover, urbanization is one of the greatest forces of environmental change affecting the world today and is seen as a driving force of climate change. By measuring the disease presence and physiology of different pollinators in urban, suburban, agricultural, and natural ecosystems, we have provided insights into the relative effects of environment on several important factors that influence their productivity and health. We are also investigating the status of feral honey bee populations in the U.S. by re-sampling non-managed honey bee populations in the southeastern United States to determine its genetic composition and to finally address whether feral populations are truly “survivor stock” or simply recent “escaped swarms”. Major collaborators: Hannah Burrack, Steve Frank, Debbie Delaney

Oxidative stress and social immunity

Hives
Stress resistance is an important trait for honey bee health and performance that needs to be evaluated and selected for to sustain honey bees for an increasingly managed, industrial life-style. The oxidative damage and its consequences are a function of the exposure and the internal defenses against oxidative stress. Our research has attempted to quantify variability in oxidative stress and lifespan within and among honey bee colonies. Similarly, we also examine the social context of stress- and parasite-resistance mechanisms to better understand the evolution of physiological and behavioral immunity in social systems. These group-level defenses—known as social immunity—emerge from collective behaviors of individuals that arise to resist persistence of infection at the colony level. Our research takes an integrative approach to understand individual immunity, genetic diversity, and social immune defenses on group-level fitness. Major collaborators: Olav Rueppell, Mimi Strand, Mike Simone-Finstrom

Parasite and pathogen IPM

Disease
Integrated pest management (IPM) is a central tenet of agricultural and apicultural practice. Implementing an IPM strategy for economically important pests, most noably varroa mites (Varroa destructor), is therefore critical for beekeeping to be sustainable.We have investigated the Russian stock for their efficacy at lowering mite levels across different habitats in North Carolina and management practices. We are also investigating various biopesticides on the control of the damaging larval phase of Small Hive Beetles (SHB). Finally, as participating members of the Bee Informed Partnership, we have been working with beekeepers and BIP tech-transfer teams to quantify baseline levels of economically important viruses to determine their effects on colony health. Major collaborators: Dennis vanEngelsdorp, Dick Rogers

Queen reproductive quality

Queen
We investigate the “mating health” of honey bee queens by looking at the physical quality, insemination success, and mating numbers of queen bees. To measure mating number, we use PCR using microsatellite markers to genotype workers in colonies and infer the mating numbers of individual queens. We also manipulate queen quality by rearing older worker larvae, which has provided insights into the link between queen quality and colony productivity, mating success, and larval development. Our integrative research program is also identifying causative genetic factors affecting honey bee queen quality and production in an effort to increase both. Our research has demonstrated marked variation among honey bee strains for traits associated with queen quality and production. We also have an extended interest in the dramatic worker-queen interactions during queen production in honey bee colonies. Major collaborators: Stan Schneider, Tim Linksvayer, Mike Simone-Finstrom, Klaus Hartfelder, Juliana Rangel