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Molecular
Genetics of the Ethylene Signal
Transduction Pathway in Arabidopsis
The main goal of our research
is to uncover the molecular mechanisms that underlie ethylene signaling
and response in Arabidopsis. In other words, to understand how
the binding of this plant hormone to its receptors generates a signal,
how this signal is transmitted from these receptors to the cell nucleus
where the transcription of a large number of genes is altered, and how
these transcriptional changes are coordinated with other developmental
and hormonal cues to produce a specific response. To study this process
in all its complexity we are using a variety of genetic and genomic approaches.
If you are interested in molecular
genetics or genomics and want to participate in this research effort,
contact Jose Alonso.
Selected
Publications:
Stepanova A, Robertson-Hoyt J, Yun J, Benavente LM, Xie D, Doležal K, Schlereth A, Jürgens G, and Alonso JM. 2008. TAA1-mediated auxin biosynthesis is essential for hormone crosstalk and plant development. Cell, in press.
Chekanova JA, Gregory BD, Reverdatto SV, Chen H, Kumar R, Hooker T, Yazaki J, Li P, Skiba N, Peng Q, Alonso J, Brukhin V, Grossniklaus U, Ecker JR, Belostotsky DA. 2007. Genome-Wide High-Resolution Mapping of Exosome Substrates Reveals Hidden Features in the Arabidopsis Transcriptome. Cell 131:1340-1353
O’Malley RC, Alonso JM, Kim CJ, Leisse TJ, and & Ecker JR. 2007. An adapter ligation-mediated PCR method for high-throughput mapping of T-DNA inserts in the Arabidopsis genome. Nature Protocols. 2:2910-17
Li H, He Z, Lu G, Lee SC, Alonso J, Ecker JR, Luan S. 2007. WD40 Domain Cyclophilin Interacts with Histone H3 and Functions in Gene Repression and Organogenesis in Arabidopsis. Plant Cell. 19:2403-16
Stepanova AN, Yun J, Likhacheva AV, Alonso JM. 2007. Multilevel Interactions between Ethylene and Auxin in Arabidopsis Roots. Plant Cell. 19:2169-85
CE, Li J, Argueso C, Gonzalez M, Lee E, Lewis MW, Maxwell BB, Perdue TD, Schaller GE, Alonso JM, Ecker JR, Kieber JJ. 2006. The Arabidopsis Histidine Phosphotransfer Proteins Are Redundant Positive Regulators of Cytokinin Signaling. Plant Cell. 18:3073-87
Sun A. Kim, Tracy Punshon, Antonio Lanzirotti, Liangtao Li, José M. Alonso, Joseph R. Ecker, Jerry Kaplan, and Mary Lou Guerinot. 2006. Localization of Iron in Arabidopsis Seed Requires the Vacuolar Membrane Transporter VIT1. Science. 314:1295-1298
Mori IC, Murata Y, Yang Y, Munemasa S, Wang YF, Andreoli S, Tiriac H, Alonso JM, Harper JF, Ecker JR, Kwak JM, Schroeder JI. 2006. CDPKs CPK6 and CPK3 Function in ABA Regulation of Guard Cell S-Type Anion- and Ca(2+)- Permeable Channels and Stomatal Closure. PLoS Biol. 4: 1749-176.
Lariguet P, Schepens I, Hodgson D, Pedmale UV, Trevisan M, Kami C, de Carbonnel M, Alonso JM, Ecker JR, Liscum E, Fankhauser C. 2006. PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. Acad Sci U S A. 103(26):10134-9
Chen JG, Ullah H, Temple B, Liang J, Guo J, Alonso JM, Ecker JR, Jones AM. 2006. RACK1 mediates multiple hormone responsiveness and developmental processes in Arabidopsis. J Exp Bot. 57: 2697-2708
A, Friso G, Alonso JM, Ecker JR, van Wijk KJ. 2006. Downregulation of ClpR2 Leads to Reduced Accumulation of the ClpPRS Protease Complex and Defects in Chloroplast Biogenesis in Arabidopsis. Plant Cell. 18: 1704-1721
Alonso JM, Ecker JR. 2006. Moving forward in reverse: genetic technologies to enable genome-wide phenomic screens in Arabidopsis. Nat. Rev. Genet. 7: 524-536
Benavente LM and Alonso JM. 2006. olecular mechanisms of ethylene signaling in Arabidopsis. Mol. BioSyst. 2:165-173
Mason MG, Mathews DE, Argyros DA, Maxwell BB, Kieber JJ, Alonso JM, Ecker JR, Schaller GE. 2005. Multiple type-B response regulators mediate cytokinin signal transduction in Arabidopsis. Plant Cell. 17:3007-18
Nagpal P, Ellis CM, Weber H, Ploense SE, Barkawi LS, Guilfoyle TJ, Hagen G, Alonso JM, Cohen JD, Farmer EE, Ecker JR, Reed JW. 2005. Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation. Development. 132:4107-18
Wilmoth JC, Wang S, Tiwari SB, Joshi AD, Hagen G, Guilfoyle TJ, Alonso JM, Ecker JR, Reed JW. 2005. NPH4/ARF7 and ARF19 promote leaf expansion and auxin-induced lateral root formation. Plant J. 43:118-30
Stepanova AN, Hoyt JM, Hamilton AA, Alonso JM. 2005. A Link between ethylene and auxin uncovered by the characterization of two root-specific ethylene-insensitive mutants in Arabidopsis. Plant Cell. 17:2230-42
Stepanova, A.N. and Alonso,
J.M. 2005. Ethylene signaling and response pathway: A unique signaling
cascade with a multitude of inputs and outputs. Physiol. Plant 123:
195-206.
Ryu, J.S., Kim, J.I., Kunkel,
T., Kim, B.C., Cho, D.S., Hong, S.H., Kim, S.H., Fernandez, A.P., Kim,
Y., Alonso, J.M., Ecker, J.R., Nagy, F., Lim, P.O., Song, P.S., Schafer,
E., and Nam, H.G. 2005. Phytochrome-specific type 5 phosphatase controls
light signal flux by enhancing phytochrome stability and affinity for
a signal transducer. Cell 120: 395-406. [pdf
file]
Okushima, Y., Overvoorde, P.J.,
Arima, K., Alonso, J.M., Chan, A., Chang, C., Ecker, J.R., Hughes, B.,
Lui, A., Nguyen, D., Onodera, C., Quach, H., Smith, A., Yu, G., and Theologis,
A. 2005. Functional genomic analysis of the AUXIN RESPONSE FACTOR gene
family members in Arabidopsis thaliana: Unique and overlapping
functions of ARF7 and ARF19. Plant Cell 17:444-4463. [pdf
file]
Liu, G., Holub, E.B., Alonso,
J.M., Ecker, J.R., and Fobert, P.R. 2005. An Arabidopsis NPR1-like
gene, NPR4, is required for disease resistance. Plant J.
41: 304-318. [pdf file]
Prigge, M.J., Otsuga, D., Alonso,
J.M., Ecker, J.R., Drews, G.N., and Clark, S.E. 2005. Class III homeodomain-leucine
zipper gene family members have overlapping, antagonistic, and distinct
roles in Arabidopsis development. Plant Cell 17:
61-76. [pdf file]
Alonso, J.M and Stepanova,
A.N. 2004. The ethylene signaling pathway. Science 306:
1513-1515. [pdf file]
Stepanova, A.N. and Alonso
J.M. 2004. The canonical ethylene signaling pathway. Sci. STKE, cm1.
Mockler, T.C., Yu, X., Shalitin,
D., Parikh, D., Michael, T.P., Liou, J., Huang, J., Smith, Z., Alonso,
J.M., Ecker, J.R., Chory, J., and Lin, C. 2004. Regulation of flowering
time in Arabidopsis by K homology domain proteins. Proc. Natl. Acad.
Sci. USA 101: 12759–12764. [pdf file]
Li, H., Johnson, P., Stepanova,
A., Alonso, J.M., and Ecker, J.R. 2004. Convergence of signaling pathways
in the control of differential cell growth in Arabidopsis. Dev. Cell
7: 193–204. [pdf file]
Page, M.L., Hamel, P.P., Gabilly,
S.T., Zegzouti, H., Perea, J.V., Alonso, J.M., Ecker, J.R., Theg, S.M.,
Christensen, S.K., and Merchant, S. 2004. A homolog of prokaryotic thiol-disulfide
transporter CcdA is required for the assembly of the cytochrome b6f complex
in Arabidopsis chloroplasts. J. Biol. Chem. 279:
32474–32482. [pdf file]
Tyler, L., Thomas, S.G., Hu,
J., Dill, A., Alonso, J.M., Ecker, J.R., and Sun, T.P. 2004. Della proteins
and gibberellin-regulated seed germination and floral development in Arabidopsis.
Plant Physiol. 135: 1008–1019. [pdf
file]
Chen, J.G., Pandey, S., Huang,
J., Alonso, J.M., Ecker, J.R., Assmann, S.M., and Jones, A.M. 2004. GCR1
can act independently of heterotrimeric G-protein in response to brassinosteroids
and gibberellins in Arabidopsis seed germination. Plant Physiol.
135: 907–915. [pdf file]
Pfund, C., Tans-Kersten, J.,
Dunning, F.M., Alonso, J.M., Ecker, J.R., Allen, C., and Bent, A.F. 2004.
Flagellin is not a major defense elicitor in Ralstonia solanacearum cells
or extracts applied to Arabidopsis thaliana. Mol Plant Microbe Interact.
17: 696–706. [pdf file]
Novillo, F., Alonso, J.M.,
Ecker, J.R., and Salinas, J. 2004. CBF2/DREB1C is a negative regulator
of CBF1/DREB1B and CBF3/DREB1A expression and plays a central role in
stress tolerance in Arabidopsis. Proc. Natl. Acad. Sci. USA 101:
3985–3990. [pdf file]
To, J.P.C., Haberer, G., Ferreira,
F.J., Deruere, J., Mason, M.G., Schaller, G.E., Alonso, J.M., Ecker, J.R.,
and Kieber, J.J. 2004. Type-A ARRs are partially redundant negative regulators
of cytokinin signaling in Arabidopsis. Plant Cell 16: 658–671.
[pdf
file]
Alonso, J.M., et al. 2003.
Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science.
301: 653-657. [pdf file]
Alonso, J.M., Stepanova, A.N.,
Solano, R., Wisman, E., Ferrari, S., Ausubel, F.M., Ecker, J.R. 2003.
Five components of the ethylene-response pathway identified in a screen
for weak ethylene-insensitive mutants in Arabidopsis. Proc Natl Acad
Sci U S A 100:2992-7. [pdf file]
Larkin, R.M., Alonso, J.M.,
Ecker, J.R., Chory, J. 2003. GUN4, a regulator of chlorophyll synthesis
and intracellular signaling. Science 299:902-906. [pdf
file]
Mackey, D., Belkhadir, Y.,
Alonso, J.M., Ecker, J.R., Dangl, J.L. 2003. Arabidopsis RIN4 is a target
of the type III virulence effector AvrRpt2 and modulates RPS2-mediated
resistance. Cell 112:379-89. [pdf
file]
Monte, E., Alonso, J.M., Ecker,
J.R., Zhang, Y., Li, X., Young, J., Austin-Phillips, S., & Quail, P.H.
2003. Isolation and characterization of phyC mutants in Arabidopsis reveals
complex crosstalk Between phytochrome signaling pathways. Plant Cell
15: 1962-1980. [pdf file]
Stepanova, A.N. and Alonso,
J.M. 2003. T-DNA mutagenesis in Arabidopsis. In Plant Functional
Genomics. (Grotewold, E., ed.) Humana Press, Totawa, New Jersey.
Strand, A., Asami, T., Alonso,
J., Ecker, J.R., and Chory, J. 2003. Mg-protoporphyrinIX is a signalling
molecule involved in retrograde communication between the chloroplast
and nucleus. Nature 421:79-83. [pdf
file]
Ullah, H., Chen, J.G., Temple,
B., Boyes, D.C., Alonso, J.M., Davis, K.R., Ecker, J.R., Jones, A.M. 2003.
The beta-subunit of the Arabidopsis G protein negatively regulates auxin-induced
cell division and affects multiple developmental processes. Plant Cell
15:393-409. [pdf file]
**These
authors contributed equally to this work.
For more information contact:
Dr. Jose M. Alonso
Department of Genetics
N.C. State University
Raleigh, NC 27695-7614
Tel: (919) 515-5729
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