Plant Parasitic
Nematodes

AN INTRODUCTION

 
 
 
 
 

Webmaster: Daniel Snyder

Last Edited: May 30, 2002

 

Genus Globodera

The Cyst Nematode

Most important species: G. rostochiensis and G. pallida

  • limited host range and mainly found in temperate climates
  • reproduce by amphimixis
  • form round cysts (female body) that hardens and encloses eggs
  • second most damaging nematode pathogen on crops
  • 13 species identified
  • primary impact on solanaceous plants(potato, tomato, eggplant, tobacco)
  • typically have one generation per year(primary inoculum)

Globodera rostochiensis (potato cyst "golden nematode")
Globodera pallida (potato cyst)
Globodera tabacum (tobacco cyst)

  • subspecies solanacearum
  • subspecies tabacum
  • subspecies virginae

Life Cycle

  • J2 cuts slit in egg shell with stylet(no enzymes involved)
  • J2 migrates to root zone of elongation and penetrates to vascular cylinder
  • feeding site(syncytium) is initiated and J2 molts to sedentary J3
  • adult female produces 200-600 eggs that mature in the cyst
  • some species produce an egg mass for spontaneous hatching
  • eggs retained in cysts overwinter or survive up to 30 years(G. pallida)
  • hatching is often stimulated by hatching factors (root exhudates)

Hots parasite interactions

  • intracellular migration of J2 causes necrosis mechanically and with cellulases
  • syncitia are formed in pericyle and endodermal cells by dissolution of cell walls
  • this syncytial initial is formed by the excretion of esophageal compounds
  • multinucleate cells result from the coalescence of neighboring cells
  • nuclei enlarge and cytoplasm becomes granulated
  • the syncitium shows increased metabolism in response to the nematode which is acting like a nutrient sink in the infected roots
  • feeding tubes form from nematode and plant secretions in the syncitium at the distal tip of the stylet, but are not enveloped by endoplasmic reticulum as in root-knot feeding tubes
  • a feeding plug is also formed at the point of cell wall penetration
  • no galling due to the lack of hyperplasia and hypertrophy
  • degradation of the syncitium results in considerably more necrosis than root-knot

 

University of Nebraska-Lincoln site for G. pallida and G. rostochiensis