Publications:(Last
Five Years)
Haigler,
C.H. In Press, 2006. Establishing the cellular and biophysical
context of cellulose synthesis. In: T. Hayashi, ed. The
Science and Lore of the Plant Cell Wall: Biosynthesis,
Structure and Function, Universal Publishers: BrownWalker
Press
Udall JA, Swanson
JM, Haller K, Rapp RA, Sparks ME, Hatfield J, Yu Y, Wu
Y, Dowd C, Arpat AB, Sickler BA, Wilkins TA, Guo JY, Chen
XY, Scheffler J, Talierco E, Turley R, McFadden H, Payton
P, Allen R, Zhang D, Haigler C, Wilkerson C, Suo J, Schulze
SR, Pierce ML, Essenberg M, Kim H, Llewellyn DJ, Dennis
ES, Kudrna D, Wing R, Paterson AH, Soderlund C, Wendel
JF. In press, 2006. A global assembly of cotton ESTs.
Genome Research
Singh B, Haley
L, Nightengale J, Kang WH, Haigler CH, Holaday AS. 2005.
Long-term night chilling of cotton, Gossypium hirsutum,
does not result in reduced CO2 assimilation. Functional
Plant Biology32: 655-666
Haigler, C.H.,
Zhang, D., Wilkerson, C.G. 2005. Biotechnological improvement
of cotton fiber maturity. Physiologia Plantarum 124: 285-294.
Roberts, A.W.,
Frost, A.O., Roberts, E.M., and Haigler, C.H. 2004. Roles
of microtubules and cellulose microfibril assembly in
the localization of secondary cell wall synthesis in developing
tracheary elements. Protoplasma 224: 217-229.
Martin, L.K.
and C.H. Haigler. 2004. Cool temperature hinders flux
from glucose to sucrose during cellulose synthesis in
secondary wall stage cotton fibers. Cellulose 11: 339-349.
Zhang, D., M. Hrmova, C.-H. Wan, C. Wu, J. Balzen, W.
Cai, J. Wang, L.D. Densmore, G.B. Fincher, H. Zhang and
C.H. Haigler. 2004. Members of a new group of chitinase-like
genes are expressed preferentially in cotton cells with
secondary walls. Plant Molecular Biology 54:353-372.
Kiedaisch,
B.M., Blanton, R. L., Haigler, C. H. 2003. Characterization
of a novel cellulose synthesis inhibitor. Planta 217:
922-930.
Haigler CH.
2003. Progress and emerging questions in understanding
cellulose biogenesis. Proceedings, Vol. 1: 12th International
Symposium on Wood and Pulping Chemistry, Univ. Wisconsin-Madison,
pp. 9-16.
Salnikov, V.,
Grimson, M.J., Seagull, R.W., Haigler, C.H. 2003. Localization
of sucrose synthase and callose in freeze substituted,
secondary wall stage, cotton fibers. Protoplasma 221:
175-184.
Delmer,
D.P. and Haigler, C.H. 2002. The regulation of metabolic
flux to cellulose, a major sink for carbon in plants.
Metabolic Engineering 4: 22-28
Babb, V.M.
and Haigler, C.H. 2001. Sucrose phosphate synthase activity
rises in correlation with high-rate cellulose synthesis
in three heterotrophic systems. Plant Physiology 127:
1234-1242.
Haigler, C.H.,
Babb. V.M., Hwang, S., and Salnikov, V.V. 2001. Regulation
of cellulose biosynthesis in developing xylem. In Molecular
Breeding of Woody Plants, N Morohoshi and A Komamine,
eds, Elsevier Science B.V.: Amsterdam, pp. 1 – 9.
Haigler,
C.H., M. Ivanova-Datcheva, P. S. Hogan, V. V. Salnikov,
S. Hwang, L. K. Martin, and Delmer, D.P. 2001. Carbon
partitioning to cellulose synthesis. Plant Molecular
Biology 47: 29-51.
Salnikov, V.V,
Grimson, M.J., Delmer, D.P., and Haigler, C.H. 2001. Sucrose
synthase localizes to cellulose synthesis sites in tracheary
elements. Phytochemistry 57: 823-833.
Haigler,
C.H., Cai, W.X., Gannaway, J.G., Grimson, M.J., Hequet,
E.F., Holaday, A.S., Huang, J.-Y., Jaradat, T.T., Jividen,
G.J., Krieg, D.R., Martin, L.K., Nagarur, S., Salnikov,
V.V., Strauss, R.E., Tummala, J., Wan, C.H., Wu, C., Wyatt,
B.G., and Zhang, H. 2000. Optimizing secondary wall synthesis
in cotton fibers. In: C.R. Benedict, ed., Genetic Control
of Cotton Fiber and Seed Quality, Cotton Incorporated:
Cary, NC, pp. 147-165.
Roberts,
A.W., S.G. Donovan, and C.H. Haigler. 1997. A secreted
factor induces cell expansion and formation of metaxylem-like
tracheary elements in xylogenic suspension cultures of
Zinnia Plant Physiology 115: 683-692.
Grimson,
M.J., C.H. Haigler, and R.L. Blanton. 1996. Cellulose
microfibrils, cell motility, and plasma membrane organization
change in parallel during culmination in Dictyostelium
discoideum. Journal of Cell Science 109:3079 - 3087.
Haigler,
C.H. and R.L. Blanton. 1996. (Invited Commentary) New
hope for old dreams: Evidence that plant cellulose synthase
genes have finally been identified. Proceedings of the
National Academy of Sciences USA 93: 12082-12085.
Taylor,
J.G., C.H. Haigler, D.G. Kilburn, and R.L. Blanton. 1996.
Detection of cellulose with improved specificity using
laser-based instruments. Biotechnic and Histochemistry
71: 215-223.
Blanton,
R.L. and C.H. Haigler. 1996. Cellulose biosynthesis,
in M. Smallwood, J.P. Knox, and D.J. Bowles, eds.
Membranes: Specialized Functions in Plants, BIOS Scientific
Publishers, Oxford, UK, p. 57-75.
Amor,
Y., C. H. Haigler, S. Johnson, M. Wainscott, and D.P.
Delmer. 1995. A membrane-associated form of sucrose synthase
and its potential role in synthesis of cellulose and callose
in plants. Proceedings of the National Academy of Sciences
USA 92: 9353-9357.
Haigler,
C. H., J. G. Taylor, and L.K. Martin. 1994. Temperature
dependence of fiber cellulose biosynthesis: Impact on
fiber maturity and strength. in Proceedings of the Biochemistry
of Cotton Workshop, Galveston, TX, Sept. 28-30, Cotton
Incorporated, Raleigh, NC. p. 95-100.
Haigler,
C.H. 1994. From signal transduction to biophysics: tracheary
element differentiation as a model system. (Invited Commentary)
International Jourrnal of Plant Science 155: 248-250.
Roberts,
A.W. and C.H. Haigler. 1994. Cell expansion and tracheary
element differentiation are regulated by extracellular
pH in mesophyll cultures of Zinnia elegans L. Plant Physiology
105: 699-706.
Xie,
W., N.L. Trolinder, and C.H. Haigler. 1993. Cool temperature
effects on cotton fiber initiation and elongation clarified
using in vitro cultures. Crop Science 33: 1258-1264.
Taylor,
J.G. and C.H. Haigler. 1993. Patterned secondary wall
assembly in tracheary elements occurs in a self-perpetuating
cascade. Acta Botanica Neerlandica 42: 153-163.
Taylor,
J.G., T.P. Owen, Jr., L.T. Koonce, and C.H. Haigler. 1992.
Dispersed lignin in tracheary elements treated with cellulose
synthesis inhibitors provides evidence that molecules
of the secondary cell wall mediate wall patterning. The
Plant Journal 2: 959-970.
Haigler,
C.H. 1992. The crystallinity of cotton cellulose in relation
to cotton improvement. Proc. Cotton Fiber Cellulose: Structure,
Function, and Utilization Conference, National Cotton
Council of America: Memphis, TN., p. 211-225.
Roberts,
E.M., R.R. Nunna, J.Y. Huang, N.L. Trolinder, and C.H.
Haigler. 1992. Effects of cycling temperatures on fiber
metaBOlism in cultured cotton ovules. Plant Physiology
100: 979-986.
Roberts,
A.W. and C.H. Haigler. 1992. Methylxanthines reversibly
inhibit tracheary element differentiation in suspension
cultures of Zinnia elegans. Planta 186: 586-592.
Roberts,
A.W., L.T. Koonce, and C.H. Haigler. 1992. A simplified
medium for in vitro tracheary element differentiation
in mesophyll cells from Zinnia elegans. Plant Cell, Tissue,
and Organ Culture 28: 27-35.
Shang,
X.M., J.Y. Huang, C.H. Haigler, and N.L. Trolinder. 1991.
Buffer capacity of cotton cells and effects of extracellular
pH on growth and somatic embryogenesis in cotton cell
suspensions. In vitro Cellular and Developmental Biology
27P: 147-152.
Haigler,
C.H, N.R. Rao, E.M. Roberts, J.Y. Huang, D.R. Upchurch,
and N.L. Trolinder. 1991. Cultured cotton ovules as models
for cotton fiber development under low temperatures. Plant
Physiology 95: 88-96.
Haigler,
C.H. 1991. The relationship between polymerization and
crystallization in cellulose biogenesis. in C. H. Haigler
and P. Weimer, eds., Biosynthesis and Biodegradation of
Cellulose, New York: Marcel Dekker, pp. 99-124.
Roberts,
A.W. and C.H. Haigler. 1990. Tracheary-element differentiation
in suspension cultures of Zinnia requires uptake of extracellular
Ca2+. Experiments with calcium-channel blockers and calmodulin
inhibitors. Planta 180: 502-509.
Roberts,
A.W. and C.H. Haigler. 1989. Rise in chlorotetracycline
fluorescence accompanies tracheary element differentiation
in suspension cultures of Zinnia. Protoplasma. 152: 37-45.
Haigler,
C.H. and A.W. Roberts. 1989. Structural aspects of tracheary
element differentiation in suspension cultures of Zinnia
elegans. In Proceedings of the 47th Annual Meeting of
the Electron Microscopy Society of America, G.W. Bailey,
ed. San Francisco Press: San Francisco, CA, pp. 768-769.
Haigler,
C.H. and H. Chanzy. 1989. Electron diffraction analysis
of altered cellulose: Implications for mechanisms of biogenesis.
In Cellulose and Wood: Chemistry and Technology, C. Schuerch,
ed., John Wiley and Sons: New York, pp. pp. 493-506.
Haigler,
C.H. and H. Chanzy. 1988. Electron diffraction analysis
of the altered cellulose synthesized by Acetobacter xylinum
in the presence of fluorescent brightening agents and
direct dyes. Journal of Ultrastructure and Molecular Structure
Research 98: 299-311.
Haigler,
C.H. and R.M. Brown, Jr. 1986. Transport of rosettes from
the Golgi apparatus to the plasma membrane in isolated
mesophyll cells of Zinnia elegans during differentiation
to tracheary elements in suspension culture. Protoplasma
134: 111-120.
Raikhel,
N., B.A. Palevitz, and C.H. Haigler. 1986. Abscisic acid
control of lectin accumulation in wheat seedlings and
callus cultures. Effects of exogenous ABA and Fluridone.
Plant Physiology 80: 167-171.
Haigler,
C.H. 1985. The functions and biogenesis of native cellulose.
In Cellulose Chemistry and its Applications, S.H. Zeronian
and T.P. Nevell, eds., Ellis Horwood: Chichester, England,
pp. 30-83.
Brown,
R.M., Jr., C. Haigler, J. Suttie, A. White, E. Roberts,
C. Smith, T. Itoh, and C. Cooper. 1983. The biosynthesis
and degradation of cellulose. Applied Polymer Symposium
37: 33-78.
Brown,
R.M., Jr., C.H. Haigler, and K.M. Cooper. 1982. Experimental
induction of altered non-microfibrillar cellulose. Science
218: 1141-1142.
Roberts,
E.M., R.W. Seagull, C.H. Haigler, and R.M. Brown, Jr.
1982. Alteration of cellulose microfibril formation in
eukaryotic cells: Calcofluor White interferes with microfibril
assembly and orientation in Oocystis apiculata. Protoplasma
113: 1-9.
Haigler,
C.H., A.R. White, R.M. Brown, Jr., and K.M. Cooper.
1982. Alteration of in vivo cellulose ribbon assembly
by carboxymethylcellulose and other cellulose derivatives.
Journal of Cell Biology 94: 64-69.
Haigler,
C.H. and M. Benziman. 1982. Biogenesis of cellulose I
microfibrils occurs by cell-directed self-assembly in
Acetobacter xylinum. In Cellulose and Other Natural Polymer
Systems, R.M. Brown, Jr., ed., Plenum: New York, pp. 273-296.
Haigler,
C.H. and R.M. Brown, Jr. 1981. Probing the relationship
of polymerization and crystallization in the biogenesis
of cellulose I. The Ekman-Days (1981) 5: 14-16 (Proceedings
of the International Symposium on Wood and Pulping Chemistry,
Stockholm, Sweden, June 9-12). Swedish Paper Chemistry
Institute: Stockholm, Sweden.
Benziman,
M., C.H. Haigler, R.M. Brown, A.R. White, and K.M. Cooper.
1980. Cellulose biogenesis: Polymerization and crystallization
are coupled processes in Acetobacter xylinum . Proceedings
of the National Academy of Science USA 77: 6678-6682.
Haigler,
C.H., R.M. Brown, Jr., and M. Benziman. 1980. Calcofluor
White ST alters the in vivo assembly of cellulose microfibrils.
Science 210: 903-906.
PATENTS
GRANTED:
Haigler,
C.H. and Holaday, A.S. October 29, 2002. U.S. Patent 6,472,588.
Transgenic cotton plants with altered fiber characteristics
transformed with a sucrose phosphate synthase nucleic
acid (16 claims).
GRADUATE
STUDENTS SUPERVISED TO DEGREE COMPLETION (OR IN PROGRESS):
2003
Ph.D. student, Utku Avci (January 2003 - present)
1999-2002
M.S. student, Brett Kiedaisch, M.S. granted, 8/02,
Thesis:
“Characterization of an experimental cellulose
synthesis inhibitor”. Next employment: Technician
at the USDA/ARS Plant Stress and Water Conservation
Unit, Lubbock TX.
1997-2002
Ph.D. student, Sangjoon Hwang, Ph.D. granted, 8/02, Dissertation:
“Molecular cloning and characterization of cellulose
synthase genes expressed during tracheary element differentiation
in cultures of Zinnia elegans.” Next employment:
Postdoctoral Research Associate, Texas Tech University.
1992-1999
Ph.D. student, L. Kirt Martin, Ph.D. granted, 5/99,
Dissertation:
“Cool-temperature-induced changes in metaBOlism
related to cellulose synthesis in cotton fibers”.
This dissertation was highlighted in the 1999 report
of the Texas Tech University Graduate School. Current
Employment: Professor, Lubbock Christian University,
Lubbock TX.
1996-1998
M.S. student, Trina Muehring, M.S. thesis granted, 5/98,
Thesis: Development of methodology for freeze substitution
and immunolocalization in differentiating tracheary elements
of Zinnia elegans. Next employment: Computer software
development, private industry.
1994-1995
M.S. student, Jyothi Tummala, , M.S. granted 5/96, Thesis:
"Response of sucrose phosphate synthase activity
to cool temperatures in cotton fiber"
Next Employment: Laboratory Technician, Kettering Labs:
New York, NY.
1991-1995
Ph.D. student, Linda T. Koonce (now Trolinder), Ph.D.
granted, 8/95. Dissertation: "The isolation and
characterization of partial cDNAs associated with
in vitro tracheary element formation." Current
employment, Elite Events Coordinator, Bayer Crop
Science.
1988-1993
Ph.D. student, Wuzi Xie. Ph.D. granted, December 1993.
1988, Texas Tech Institute for Biotechnology Research
Fellow. Dissertation: "Morphological and biochemical
characterization of cool temperature hindrance of fiber
elongation of cotton using ovules cultured in vitro as
a model system."
Current employment: QA/QC Lab, Bayer CropScience.
1989-1993
Ph.D. student, Jim Taylor. Ph.D. granted, December, 1993.
Dissertation: "The investigation of secondary wall
deposition in differentiating Zinnia cell suspension cultures".
1989-90, Texas Tech Institute for Biotechnology Research
Fellow. Winner of the Robert I. Larus award for best presentation
by a graduate student, SWARM-AAAS, 1991; Winner of a best
poster award at Cellulose '91, New Orleans and AAAS, Chicago,
1992.
Current employment: Associate Professor of Biology, Ouachita
Baptist University, Arkadelphia, AR.
1987-1990
Ph.D. student, Alison Roberts. Ph.D. granted, May 1990.
Dissertation: "The role of calcium ions in the differentiation
of tracheary elements from isolated cells in suspension
culture". Recipient of Outstanding Graduate Student
Research Award, Dept. of Biological Sciences, 1991.
Current employment: Professor of Biological Sciences,
University of Rhode Island, Kingston, RI
INVITED
RESEARCH SEMINARS (last 10 years):
2005
Biosynthesis of Plant Cell Walls, Pacific Grove, CA, 8/4-7,
“Secondary wall stage cotton fiber provides an optimum
platform for analysis of gene expression related to cellulose
synthesis”
Private
industry, 9/12, “Molecular characteristics of cotton
fiber”.
NC
Plant Molecular Biology Retreat, 9/30 – 10/2, “Cotton
fiber as an optimum platform for single cell genomics
related to cell expansion and cell wall synthesis”
Department
of Horticulture, NCSU, 10/24, “Cellular, metabolic,
and environmental control of cellulose synthesis
in cotton fiber”
2004
American Chemical Society, Anselme Payen Award Symposium
honoring D. Delmer, Co-organizer and speaker, 3/28-30,
Anaheim, CA
Southern
Section of the American Society of Plant Biologists, “Biotechnological
improvement of cotton fiber maturity”, 3/6-8, Lafayette,
LA
USDA
Cotton Biotechnology Workshop, “Gene expression
in cotton fiber”, 10/4-5, New Orleans, LA
USDA
Nanotechnology for the Forest Products Industry Workshop,
Co-leader of and speaker in the working group on the Nanostructure
of Plant Cell Walls, 10/17-19, Lansdowne, VA
2003
12th International Symposium on Wood and Pulping Chemistry,
“Progress and emerging questions in understanding
cellulose biogenesis”
Fibre
Wall, 5th workshop of COST Action E20, Helsinki Finland,
“Cellulose and lignin biosynthesis: xylem vessel
formation in vitro”
2002
North Texas State University, “Environmental regulation
of cotton fiber cellulose synthesis”
Cotton
Incorporated, Raleigh, NC. Cotton Genetics Working Group
Meeting, “Genetic engineering of secondary wall
deposition in cotton fiber”
North
Carolina State University at Raleigh, “Control of
cellulose synthesis and its cool temperature response
by sucrose synthase and sucrose phosphate synthase”
2001
International Wood Biotechnology Symposium, Narita, Japan,
“Regulation of cellulose biosynthesis in developing
xylem”
University
of Dundee, Division of Environmental and Applied Biology,
“Control of cellulose synthesis and its cool temperature
response by sucrose synthase and sucrose phosphate synthase”
2000
219th ACS National Meeting, Cellulose, Paper, and Textile
Division, “Regulation of cellulose synthesis in
differentiating tracheary elements of Zinnia elegans”
Gordon
Conference on Plant Cell Walls, Meriden, NH, “Structure
of cellulose-synthesizing complexes”
Cotton
Incorporated Conference on Genetic Improvement of Cotton,
San Antonio, TX, “Optimizing secondary wall synthesis
in cotton fiber”
1999
North Carolina State University, “Mechanisms of
tracheary element differentiation”
1998
Texas A&M University, “Genetic engineering for
improved cool tolerance in cotton fiber development”
Plant
Genetic Systems, Gent, Belgium, “Strategies for
improving cool temperature tolerance of cotton fiber development”
Centre
de Recherches sur les Macromolecules Vegetales, Grenoble,
France, “Cool temperature sensitivity of cellulose
biosynthesis in cotton fibers”
Centre
de Biologie et Physiologie Vegetales, Toulouse, France,
“Cellular regulation of secondary wall patterning
in differentiating tracheary elements”
XIX
International Carbohydrate Symposium, San Diego, CA “Response
of cellulose biosynthesis in cotton fibers to cool
temperature stress”
Cotton
Incorporated Conference on Cotton Biochemistry and
Biotechnology, Galveston, TX, “Cool-temperature-induced
changes in metabolism related to cellulose synthesis
in cotton fibers”
1997
Gordon Conference on Plant Cell Walls, Tilton, NH, “Cellular
determinants of secondary wall patterning in differentiating
tracheary elements”
Virginia
Polytechnical Institute, Blacksburg, VA, “Cellular
determinants of secondary wall patterning in differentiating
tracheary elements”
POPULAR
ARTICLES RELATED TO RESEARCH:
News
Release, North Carolina State University, “Professor
brings new ways of studying improvements to cotton plants”,
2003.
Co-authored
article: C.H. Haigler and R.L. Blanton. 1999. Cellulose:
You're surrounded by it, but did you know it was there?
LubBOck Magazine: December 1999, pp. 42 - 46.
Featured
faculty member in M. Gustafson. 1992. "A piece of
tradition." Texas Techsan Magazine 45 (1): 9.
Featured
faculty member in C. Patterson. 1991. "Biotechnology:
Piecing together the genetic puzzle." Vistas. Texas
Tech Research 1(2): 16-20.
Research
featured in an educational video tape, "Cotton, exploring
the inner frontiers." Copyright 1991, Cotton Incorporated
and Texas Tech University.
Research
featured in "Cotton Incorporated research seed
money multiplies to benefit producers." Cotton
Leader. February 1991. Cotton Board: Memphis, TN.
Research
featured in "U.S. cotton in action." Cotton
Farming 11(5): 22, 1990
