fermentation equipment, people


Dr. José M. Bruno-Bárcena - Associate Professor

Bioprocessing and Fermentation, Gram positive organisms



Research Interests

There is a critical need for the systematic identification and testing of new gene modulated states that participate in the enhancement of cellular stress resistance. In our laboratory we use the new era of genomic tools that make it possible to identify point mutations, insertions, deletions and/or translocations that have occurred in offspring strains following defined selection processes. In the near future, this comparative approach will provide valuable insights for identifying gene combinations and biochemical pathways that play a role in microbial metabolism and this information may be applied to further improve production using strategic strain modifications. Our primary research interests are the study of Gram positive organisms and we investigate different, but complementary, areas of study with a focus on functional probiosis and bioenergy. Thus far our bioprocessing research has generated technology, peer-reviewed publications, and intellectual property to be directly implemented and translated into the generation of biofuels and functional foods. We have dedicated significant amounts of time towards implementing these new overlapping bioprocessing research areas that target prime university interests.


Selected Publications

WJ Sandoval, S Makwana, Mari S. Chinn, MR Thon, MA Azcárate-Peril and JM Bruno-Bárcena (accepted for publication). Comparative phenotypic analysis and genome sequence of Clostridium beijerinckii SA-1, an offspring of NCIMB 8052. Microbiology, Society for General Microbiology

JM Bruno-Bárcena, Chinn MS, Grunden AM. (2013) Genome Sequence of the Autotrophic Acetogen Clostridium autoethanogenum JA1-1 Strain DSM 10061, a Producer of Ethanol from Carbon Monoxide. Genome Announc. 2013 Aug 15;1(4). doi:pii: e00628-13. 10.1128/genomeA.00628-13.

Noar JD, Bruno-Bárcena JM. (2013) Complete genome sequences of Azotobacter vinelandii wild-type strain CA and tungsten-tolerant mutant strain CA6. Genome Announc. 1(3):e00313-13. doi:10.1128/genomeA.00313-13.

S.F. Dagher, M.A. Azcarate-Peril and Bruno-Bárcena JM (2013) Heterologous expression of a bioactive β-hexosyl-transferase, an enzyme producer of prebiotics, from Sporobolomyces singularis. Appl Environ Microbiol. 79(4):1241-9

M. Andrea Azcarate-Peril, Michael Sikes, and Jose M. Bruno-Bárcena (2011) The intestinal microbiota, gastrointestinal environment and colorectal cancer: A putative role for probiotics in prevention of colorectal cance? Am J Physiol Gastrointest Liver Physiol. 2011 Sep;301(3):G401-24.

Heluane H, Evans MR, Dagher SF and Bruno-Bárcena JM (2011) Meta-Analysis and Functional Validation of Nutritional Requirements of Solventogenic Clostridia Growing under Butanol Stress Conditions and Coutilization of D-Glucose and D-Xylose. App. Environ. Microbiol. 77(13):4473-85.

J.M. Bruno-Bárcena, M.A. Azcárate-Peril, and Hosni M. Hassan (2010) Role of antioxidant enzymes in bacterial resistance to organic acids. Appl. Environ. Microbiol. 76: 2747-2753

Dagher SF, Ragout AL, Siñeriz F, Bruno-Bárcena JM (2010) Cell Immobilization for Production of Lactic Acid: Biofilms do it naturally. Advances in Applied Microbiology - Volume 71 / ISBN 978-0-12-3809933

Carroll IM, Andrus JM, Bruno-Barcena JM, Klaenhammer TR, Hassan HM, Threadgill DS (2007) The Anti-inflammatory Properties of Lactobacillus gasseri Expressing Manganese Superoxide Dismutase (MnSOD) Using the Interleukin 10-Deficient Mouse Model of Colitis. Am J Physiol Gastrointest Liver Physiol. 293:G729-38.

E. Pico-Marco, J.L. Navarro, J.M. Bruno-Bárcena (2006) A closed loop exponential feeding law: invariance and global stability analysis. J. Process Control. 16:395-402

M. Andrea Azcárate-Peril, J.M. Bruno-Bárcena, Hosni M. Hassan, T.R. Klaenhammer (2006) Transcriptional Analysis of Oxalyl-CoA Decarboxylase and Formyl-CoA Transferase Genes from Lactobacillus acidophilus. Appl. Environ. Microbiol. 72: 1891-1899

R. Pérez-Torrado, J.M. Bruno-Bárcena and E. Matallana (2005) Monitoring stress related genes during the process of biomass propagation of Saccharomyces cerevisiae strains used for wine making. Appl. Environ. Microbiol. 71: 6831-6837

J.M. Bruno-Bárcena, M.A. Azcárate-Peril, Todd R. Klaenhammer and Hosni M. Hassan (2005) Marker-free Chromosomal Integration of the Manganese Superoxide Dismutase Gene (sodA)from Streptococcus thermophilus into Lactobacillus gasseri. FEMS Microbiol. Lett. 246: 91-101

J.M. Bruno-Bárcena, J.M. Andrus, S. Libby, T.R. Klaenhammer and H.M. Hassan (2004) Expression of a Heterologous Manganese Superoxide Dismutase Gene in Intestinal lactobacilli provides protection against hydrogen peroxide toxicity. Appl. Environ. Microbiol. 70: 4702-4710

J.M. Bruno-Bárcena, M.E. Lucca, F. Siñeriz and D. Ramón (2002) pH regulation of enzyme production in Aspergillus nidulans growing in aerobic carbon-limited batch fermentor. Biotechnol. Lett. 24: 567-572

G. Raya Tonetti, P. R. Córdoba, J.M. Bruno-Bárcena, F. Siñeríz and N. I. Perotti (1999) Fluidized bed ion exchange for improving recovery of lactic acid from fermentation. Biotechnol. Tech. 13: 201-205

J.M. Bruno-Bárcena, A.L. Ragout, P.R. Córdoba and F. Siñeríz (1999) Continuous production of L(+) lactic acid by Lactobacillus casei in two-stage systems. Appl. Microbiol. Biotechnol. 51: 316-324

J. M. Bruno-Bárcena, F. Siñeríz, D. González de Llano, A. Rodríguez and J.E. Suárez (1998) Chemostat production of  plantaricin c by Lactobacillus plantarum LL 441. Appl. Environ. Microbiol. 64: 3512-3514

J.M. Bruno-Bárcena, M.A. Azcárate, A.L. Ragout, G. Font de Valdez, R.R. Raya and F. Siñeríz (1998) Fragile cells of Lactobacillus casei suitable for plasmid DNA isolation. Biotechnol. Tech. 12: 97-99

S. C. Ginés, J. M. Bruno–Bárcena, A. Ragout, G. F. de Valdez (1997) Effect of the carbon source on the fermentation balance of Lactobacillus reuteri. Microbiologie-Aliments-Nutrition. 15: 23-27

G.F. de Valdez, A.L. Ragout, J.M. Bruno-Bárcena, H. Diekmann, and F. Sineriz (1997) Shifts in the pH affect the maltose glycerol cofermentation by Lactobacillus reuteri. Biotechnol. Lett. 19: 645-649

Book Chapters:

J.M. Bruno-Bárcena, E. Picó-Marco, J.L. Navarro, and J. Picó (2007) Microbial Dynamic Transformations: Basic Concepts. Encyclopedia of Life Support Systems (EOLLS). UNESCO

J.M. Bruno-Bárcena and F. Siñeríz (2002) Industrial Biotechnology. Encyclopedia of Life Support Systems (EOLLS). UNESCO

J.M. Bruno-Bárcena, M.A. Azcárate-Peril, and F. Siñeríz (2001) Genetically modified organisms (GMO) in food products: potential risks and the reaction of the consumer. Food Protocols, 481-484. Humana press, Totowa-New Jersey.

J.M. Bruno-Bárcena, A.L. Ragout, P.R. Córdoba, and F. Siñeríz (2001) Reactor configuration for fermentation in immobilized continuous system. Food Protocols, 321-328. Humana press, Totowa-New Jersey.

J.M. Bruno-Bárcena, A.L. Ragout, and F. Siñeríz (1998) Microbial physiology applied to process optimisation: lactic acid bacteria. Advances in Bioprocess Engineering II. Vol. II, 97-110. Kluwer Academic Publishers, The Netherlands.