Dominique (Niki) Robertson
Professor of Plant Biology and Genetics
North Carolina State University
Raleigh, NC 27695-7612

Education

Ph.D. Cornell University, Ithaca, NY

M.S.1977 University of Oregon, Eugene, OR

B.S. State University of New York at Binghamton, NY

Professional Experience

2003- present: Professor, Department of Plant Biology, North Carolina State University, Raleigh, NC

2001 – present: Associate member, Department of Genetics, NCSU

2001 – 2002 Academic Coordinator, NCSU Biotechnology Program

1997 - 2003 Associate Professor. Department of Plant Biology, North Carolina State University, Raleigh, NC.

1992 2001 Coordinator for the NCSU Summer Courses in Biotechnology

1992 - 1997 Assistant Professor, Dept. of Plant Biology, North Carolina State University, Raleigh, NC.

1991 North Carolina State University, Visiting Assistant Professor

1989-1991 North Carolina State University, McKnight Postdoctoral Fellow

1986-1989 Duke University, NIH Postdoctoral Fellow

1977-1981University of Rochester, Technician/Research Associate

Scholarly and Professional Honors

Visiting Professor, Zhengzhou University, Zhengzhou, Henan Province, People’s Republic of China, May, 2002

McKnight Postdoctoral Fellowship, NCSU, 9/89 – 12/91

NIH Postdoctoral Fellowship, Duke U., 10/86 – 9/89

Phi Kappa Phi, Cornell U. 1984

Professional Associations

American Phytopathological Society, member Virology Committee 2004-2007

American Society of Plant Biology

Patents

Robertson, D. Method of suppressing gene expression in plants. US Patent 07-06-04 06759571. European patent pending.

Wyatt, S., Tsou, P.-L., BOss, W., Robertson, D. Transgenic plants with increased calcium stores. US Patent 06-22-04 06753462.

Publications:

Muangsan, N., Beclin, C., Vaucheret, H., Robertson, D. (2004) Geminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants. Plant J. 38: 1004-1014.

Hanley-BOwdoin L, Settlage SB, Robertson D (2004) Reprogramming plant gene expression – a prerequisite to geminivirus DNA replication. Mol. Plant Path. 5: 149-156.

Robertson, D. (2004) VIGS vectors for gene silencing, many targets, many tools. Ann. Rev. Plant Biol. 55: 495-519.

Muangsan, N., Robertson, D. (2004) Geminivirus vectors for transient gene silencing in plants. Methods Mol. Biol. 265: 101-116.

Nagar, S., Hanley-BOwdoin, L., and Robertson, D. (2002) Host DNA Synthesis is Induced by Geminivirus Infection of Differentiated Plant Cells. The Plant Cell. 14: 2995-3007.

Egelkrout, E., Mariconti, L., Cella, R., Robertson, D., and Hanley-Bowdoin, L. (2002) Two E2F elements regulate the proliferating cell nuclear antigen promoter during plant development. The Plant Cell. 14: 3225-3236.

Wyatt, S.E., Rashotte, A.M., Shipp, M., Robertson, D. and Muday, G. K. (2002) Mutations in the GPS loci in Arabidopsis disrupt the perception and/or signal transduction of gravitropic stimuli. Plant Physiol. 130:1426-1435.

Turnage, M.A., Muangsan, N., Peele, C.G, and Robertson, D. (2002) Geminivirus-based vector for gene silencing in Arabidopsis. Plant J. 30: 107-114.

Persson, S., Love, J., Tsou, P.L., Robertson, D., Thompson, W.F., and BOss, W.F. (2002) When a Day Makes a Difference. Interpreting Data from Endoplasmic Reticulum-Targeted Green Fluorescent Protein Fusions in Cells Grown in Suspension Culture. Plant Physiol. 128: 341-344.

Wyatt, S.E., Tsou, P.-L. and Robertson, D. (2002) Expression of the high capacity calcium-binding domain of calreticulin increases bioavailable calcium stores in plants. Transgenic Res. 11: 1-10.

Peele, C., Jordan, C.V., Muangsan, N., Turnage, M., Egelkrout, E., Eagle, P., Hanley-Bowdoin, L., and Robertson, D. (2001) Silencing of a meristematic gene using geminivirus-derived vectors. Plant J. 27: 357-66

Persson, S., Wyatt, S.E., Love, J., Thompson, W.F., Robertson, D., Boss, W.F. (2001) The Ca2+ status of the endoplasmic reticulum is altered by induction of calreticulin expression in transgenic plants. Plant Physiol. 126: 1092-104

Egelkrout, E.M., Robertson, D., and Hanley-Bowdoin, L. (2001) PCNA transcription is repressed through an E2F consensus element and activated by geminivirus infection in mature leaves. Plant Cell, 13: 1437-1452

Staman, K., Blum, U., Louws, F.,, Robertson, D. (2001) Can simultaneous inhibition of seedling growth and stimulation of rhizosphere bacterial populations provide evidence for phytotoxin transfer from plant residues in the bulk soil to the rhizosphere of sensitivespecies? J. Chem. Ecol. 27: 807-829

Kong, L.J., Orozco, B.M., Roe, J.L., Nagar, S., Ou, S., Feiler, H.S., Durfee, T., Miller, A.B., Gruissem, W., Robertson, D., and Hanley-Bowdoin, L. (2000) A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants. EMBO J. 19: 3485-3495.

Bass, H., Nagar, S., Hanley-Bowdoin, L. and Robertson, D. (2000) Chromosome condensation induced by geminivirus infection of mature plant cells. J. Cell Sci. 113: 1149-1160.

Scott A, Wyatt S, Tsou PL, Robertson D, Allen NS (1999) Model system for plant cell biology: GFP imaging in living onion epidermal cells. Biotechniques 26:1125, 1128-32

Kjemtrup, S., Peele, C., Sampson, K.S., Nguyen, L.V., Conkling, M.A., Thompson, W.F. and Robertson, D. (1998) Gene silencing from plant DNA carried by a geminivirus. Plant Journal, 14: 91-100.

Hanley-BOwdoin, L., Eagle, P.A., Orozco, B.M., Robertson, D. Settlage, S.B. (1997) Geminivirus Replication. In Biology of Plant Microbe Interactions, eds. Stacey, B., Mullin, B., Gresshoff, P.M. (Int. Soc. Mol. Plant Microbe Interactions, St. Paul) pp. 287-292.

Nagar, S. Pedersen, T., Carrick, K., Hanley-Bowdoin, L. and Robertson, D. (1995) A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells. The Plant Cell 7: 705-719.

Okpodu, C.M., Robertson, D., BOss, W.F., Togasaki, R.K. and Surzycki, S.J. (1994) Rapid isolation of nuclei from carrot suspension culture cells using a BioNebulizer. Biotechniques. 16, 154-159.

Clemente, T., Robertson, D., Isleib, T. G., Beute, M. and Weissinger, A. K. (1993) Evaluation of peanut (Arachis hypogaea L.) leaflets from mature zygotic embryos as recipient tissue for biolistic gene transfer. Transgenic Res. 1: 275-284.

Robertson, D., Weissinger, A. K., Ackley, R., Glover, S. and Sederoff, R. R. (1992) Genetic transformation of Norway spruce (Picea abies L. Karst) using somatic embryo explants by microprojectile Bombardment. Plant Molec. Biol. 19: 925-935.

Robertson, D., Boynton, J. E., and Gillham, N. W. (1990) Cotranscription of the wild-type chloroplast atpE gene encoding the CF1/CF0 epsilon subunit with the 3' half of the rps7 gene in Chlamydomonas reinhardtii and characterization of frameshift mutations in atpE. Molec. Gen. Genet. 221: 155-163.

Robertson, D., Woessner, J. P., Gillham, N. W. and Boynton, J. E. (1989) Molecular analysis of two point mutants in the chloroplast atbo gene of the green alga Chlamydomonas reinhardtii defective in assembly of the ATP synthase complex. J. Biol. Chem. 264: 2331-2337.

Boynton, J. E., Gillham, N. W., Harris, E. H., Hosler, J. P., Johnson, A. M., Jones, A. R., Randolph-Anderson, B. L., Robertson, D., Klein, T. M., Shark, K. B., and Sanford, J. C. (1988) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240: 1534-1538.

Robertson, D., Earle, E. D. and Mutschler, M. A. (1988) Increased totipotency of protoplasts from Brassica oleracea plants previously regenerated in tissue culture. Plant, Cell, Tissue and Organ Culture 14: 15-24.

Robertson, D., Palmer, J. D., Earle, E. D. and Mutschler, M. A. (1987) Analysis of organelle genomes in a somatic hybrid derived from cytoplasmic male-sterile Brassica oleracea and atrazine-resistant B. campestris. Theor. and Appl. Genet. 74: 303-309.

Robertson, D. and Earle, E. D. (1987) Nitro-blue tetrazolium: a specific stain for photosynthethic activity in protoplasts. Plant Cell Reports 6: 70-73.

Robertson, D. and Earle, E. D. (1986) Plant regeneration from leaf protoplasts of Brassica oleracea var italica CV Green Comet broccoli. Plant Cell Reports 5: 61-64.

Robertson, D. (1985) Origin and phenotypic expression of recessive chloroplast mutations in higher plants: atrazine resistance as a model system. Plant Molecular Biology Reporter 3: 99-106.

Brulfert, A., Miller, M. W., Robertson, D., Dooley, D. A. and Economou, P. (1985) A cytohistological analysis of roots whose growth is affected by a 60 Hz electric field. Bioelectromagnetics 6: 283-291.

Miller, M. W., Economou, P., Cox, C. and Robertson D. (1982) Micronuclei formation in Pisum sativum L. root meristem cells exposed to an electric field or gamma rays. Environ. and Exp. BOt. 22: 138-143.

Robertson, D., Miller, M. W. and Carstensen, E. L. (1981) Relationship of 60 Hz electric field parameters to the inhibition of growth of Pisum sativum roots. Radiat. Environ. Biophys. 19: 227-233.

Robertson, D., Miller, M. W., Cox, C. and Davis, H. T. (1981) Inhibition and recovery of growth processes in roots of Pisum sativum L. roots exposed to 60 Hz electric fields. Bioelectromagnetics 2: 239-240.

Miller, M. W., Carstensen, E. L., Robertson, D. and Child, S. Z. (1980) 60 Hz electric field parameters associated with the perturbation of a eukaryotic cell system. Radiat. Environ. Biophys. 18: 298-300.

Cox, C., Davis, H. T., Miller, M. W. and Robertson, D. (1980) Statistical analysis of root growth rate determinations. Environ. and Exp. Bot. 20: 389-396.

Books

Carson, S., and Robertson, D. (2005) Manipulation and Expression of Recombinant DNA: Core Techniques. Second edition. Elsevier, Amsterdam

Robertson, D., Shore, S.H. and Miller, D.M. (1997) Manipulation and Expression of Recombinant DNA: Core Techniques. Academic Press, San Diego CA.( Doody's 250 Best Books of the Year Selection)

Hardin, C., Pinczes, J., Riell, A., Presutti,D., Miller, M., and Robertson, D. (2001) Cloning, Gene Expression and Protein Purification. Oxford University Press, NY

Presentations at conferences, universities and institutes

“Gene silencing from homologous DNA carried by TGMV episomes.” Second International Workshop on Bemesia and Geminivirus Diseases, June 9, 1988 San Juan Puerto Rico.

“Geminivirus induction of chromosomal DNA replication” Second International Workshop on Bemesia and Geminivirus Diseases, June 8, 1988 San Juan Puerto Rico.

"Gene Silencing from Homologous DNA carried by a Geminivirus" July 21, 1996 Plant Molecular Biology Gordon Conference, New Hampton NH

"Cell and tissue specificity of geminivirus replication", September 17, 1994, First International Symposium on Geminiviruses, Almeria, Spain.

"Correlation of a chloroplast genetic locus in Chlamydomonas with the atpE gene encoding the epsilon subunit of the ATP synthase." Nov. 17, 1988, Second International Congress of Plant Molecular Biology, Jerusalem, Israel.

"Chloroplast transformation in Chlamydomonas." June 15, 1988. 39th meeting of the Tissue Culture Association, Las Vegas, Nev.

"Manipulation of organelle genomes through protoplast fusion in the genus Brassica." Colloquium on Forest and Crop Biotechnology, April 19, 1985, Syracuse University, N. Y.

Teaching

PB 480. Introduction to Plant Biotechnology, 3 credit hours, taught Spring 93, 95, 97, Fall 99, Spring 05, 06.

PB 795F Plant Science Ethics. 2 credit hours. Co-taught with Dr. Tom Wentworth, Fall 03, 05.

BIT/BO 465 Plant Tissue Culture and Transformation. 2 credit hours, taught Spring 02, 03, 04

PB 795F Ethics of Genetically Modified Organisms. 1 credit hour. Taught Fall 01.

BIT810. Core Technologies in Molecular and Cellular Biology, 4 credit hours, taught Summer 91 (as a visiting assistant professor), 92, 93, 94, 95, 96, 97, 98, 99, 00, 01

PB 624 F and G Plant Form, Function, and Development, 4 credits. Taught Fall 98, 00, 02, 03

ALS299H. 2 credit hrs, taught Spring 92, 94, 95, 96.

PB 590C Plant Cell Biology. 3 credit hours, co-taught with Dr. Nina Allen, Spring 96