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Greg OBrian
Research Interests
My current research is focused on understanding how temperature regulates aflatoxin biosynthesis. Aflatoxin is produced in cultures grown in conducive media at temperatures ranging from about 18C to 34C. Temperatures above 37C are not conducive for aflatoxin production in A. flavus. We have been studying gene expression under conducive and nonconducive temperatures to monitor the transcription levels of the aflatoxin biosynthetic genes and other genes associated with aflatoxin production.
Publications
Journal Articles
Fontes, E. B. P., B. B. Shank, R. L. Wrobel, S. P. Moose, G. R. Obrian, E. T. Wurtzel, and R. S. Boston. 1991. Characterization of an Immunoglobulin Binding-Protein Homolog in the Maize Floury-2 Endosperm Mutant. Plant Cell 3:483-496.
Bass, H. W., C. Webster, G. R. Obrian, J. K. M. Roberts, and R. S. Boston. 1992. A Maize Ribosome-Inactivating Protein Is Controlled by the Transcriptional Activator Opaque-2. Plant Cell 4:225-234.
Bass, H. W., G. R. Obrian, and R. S. Boston. 1995. Cloning and Sequencing of a 2nd Ribosome-Inactivating Protein Gene from Maize (Zea-Mays L). Plant Physiology 107:661-662.
Shi, H. P., H. Shigeta, N. Y. Yang, K. Fu, G. Obrian, and C. T. Teng. 1997. Human estrogen receptor-like 1 (ESRL1) gene: Genomic organization, chromosomal localization, and promoter characterization. Genomics 44:52-60.
Wrobel, R. L., G. R. Obrian, and R. S. Boston. 1997. Comparative analysis of BiP gene expression in maize endosperm. Gene 204:105-113.
Klintworth, G. K., J. R. Sommer, G. Obrian, L. Han, M. N. Ahmed, M. B. Qumsiyeh, P. Y. Lin, S. Basti, M. K. Reddy, A. Kanai, Y. Hotta, J. Sugar, G. Kumaramanickavel, F. Munier, D. F. Schorderet, L. El Matri, F. Iwata, M. Kaiser-Kupfer, M. Nagata, K. Nakayasu, J. F. Hejtmancik, and C. T. Teng. 1998. Familial subepithelial corneal amyloidosis (gelatinous drop-like corneal dystrophy): exclusion of linkage to lactoferrin gene. Mol Vis 4:31.
Meyers, D. M., G. Obrian, W. L. Du, D. Bhatnagar, and G. A. Payne. 1998. Characterization of aflJ, a gene required for conversion of pathway intermediates to aflatoxin. Applied and Environmental Microbiology 64:3713-3717.
OBrian, G. R., A. M. Fakhoury, and G. A. Payne. 2003. Identification of genes differentially expressed during aflatoxin biosynthesis in Aspergillus flavus and Aspergillus parasiticus. Fungal Genetics and Biology 39:118-127.
Xue, H. Q., T. G. Isleib, G. A. Payne, R. F. Wilson, W. P. Novitzky, and G. O'Brian. 2003. Comparison of aflatoxin production in normal- and high-oleic backeross-derived peanut lines. Plant Disease 87:1360-1365.
Bass, H. W., J. E. Krawetz, G. R. Obrian, C. Zinselmeier, J. E. Habben, and R. S. Boston. 2004. Maize ribosome-inactivating proteins (RIPs) with distinct expression patterns have similar requirements for proenzyme activation. Journal of Experimental Botany 55:2219-2233.
Payne, G. A., A. M. Fakhoury, K. A. Scheidegger, M. S. Price, and G. R. OBrian. 2004. Presented at the New Horizon of Mycotoxicology for Insuring Food Safety, Kagawa, Japan.
Xue, H.Q., T.G. Isleib, G.A. Payne, and G. OBrian. 2004. Evaluation of post-harvest aflatoxin production in peanut germplasm with resistance to seed colonization and pre-harvest aflatoxin contamination. Peanut Sci. 31: 124-134.
Xue, H.Q., T.G. Isleib, H.T. Stalker, G.A. Payne, and G. OBrian. 2004. Evaluation of Arachis species and interspecific tetraploid lines for resistance to aflatoxin production by Aspergillus flavus. Peanut Sci. 31: 134-141.
Xue, H. Q., T. G. Isleib, G. A. Payne, W. F. Novitzky, and G. OBrian. 2005. Aflatoxin Production in Peanut Lines Selected To Represent a Range of Linoleic Acid Concentrations. Journal of Food Protection 68:126-132.
Patents
DNA Encoding a Ribosome-Inactivating Protein (co-inventor). U.S. Patent No. 5,332,808. 1994.
DNA Encoding a Ribosome-Inactivating Protein (co-inventor). U.S. Patent No. 5,552,140. 1996.
Infectious Disease Microarray (co-inventor). Patent Pending. 2001.
Posters and Abstracts
Abstracts
Grant, D. J., G. Obrian, and C. Teng. 1996. Methylation of sequences proximal to the estrogen regulatory site in the mouse lactoferrin gene is influenced by diethylstilbestrol. Molecular Biology of the Cell 7:1722-1722.
Payne, G. A., D. M. Meyers, W. Du, G. R. OBrian, and M. P. Brown. Characterization of aflJ, a novel gene involved in aflatoxin biosynthesis. International Congress of Plant Protection,
Edinburgh, Scotland August 9-14, 1998.
Brown-Jenco, C. S., Monsanto, St. Louis, MO; G. R. OBrian, S. Sloane, and G. A. Payne, North Carolina State University, Raleigh, NC. Identification of the DNA binding site for the A. flavus AflR in the nor-promoter.
Aflatoxin Elimination Workshop. St. Louis, MO. October 25-27, 1998.
OBrian, G. R. and G. A. Payne. 2000. Differential gene expression during aflatoxin biosynthesis. Phytopathology 90: S56.
Du, W., G. R. OBrian, D. Bhatnagar, and G. A. Payne. Characterization of aflJ and Iits putative role in aflatoxin biosynthesis.
Aflatoxin Elimination Workshop. St. Louis, MO. October 25-27, 1998.
Schiffman, S. S., D. W. Wyrick, G. A. Payne, G. R. OBrian, and H. T. Nagle. Detecting Microbial Contamination Using an Electronic Nose. Seventh International Symposium olfaction and the electronic nose.
(ISOEN 2000); p 95-96. Brighten England.
Wyrick, D., S. Schiffman, G. Payne, G. OBrian, G. Van dyke, H. Allison. J. Ledbetter, H. Nagle. 2000. Testing HVAC ducts for microbial contamination using an electronic nose.
In: Proceedings of world congress on medical physics and biomedical engineering. Chicago, IL.
Scheidegger, K.A., Obrian, G.R., Keller, N.P., and Payne, G.A. 2002. Gene expression analysis in a wild type and FadA mutant of Aspergillus flavus. Annual Meeting American Phytopathological Society. July 27-31, 2002, Milwaukee, Wisconsin.
Gene expression analysis of Aspergillus flavus and A. parasiticus.
Payne, G. A., Fakhoury, A. M. and OBrian, G.R. 2002. Aflatoxin Elimination Workshop, October 23-25, 2002. San Antonio, Texas.
OBrian, G. R., Fakhoury, A. M., and Payne, G. A. 2002. Identification of genes differentially expressed during aflatoxin biosynthesis in Aspergillus flavus and Aspergillus parasiticus. Aflatoxin Elimination Workshop, October 23-25, 2002. San Antonio, Texas.
Xue, H.Q. and Isleib, Payne, G.A., OBrian, G. R., Wilson, R.F. and Novitzky, W. F. 2002. Comparison of aflatoxin production in normal- and high-oleic backcross derived peanut lines. Aflatoxin Elimination Workshop, October 23-25, 2002. San Antonio, Texas.
Screening Peanut Germplasm for Resistance to Aflatoxin Production by Aspergillus Flavus. H.Q. XUE *, T.G. ISLEIB, G.A. PAYNE, H.T. STALKER, and G. OBRIAN, Dept. of Crop Science, Box 7629, N.C. State Univ., Raleigh, NC 27695-7629 and Dept. oState Univ., Raleigh, NC 27695-7567. THIRTY-SIX ANNUAL MEETING AMERICAN PEANUT RESEARCH AND EDUCATION SOCIETY San Antonio, Texas JULY 13-16, 2004
Contact Information
grobrian@unity.ncsu.edu
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