venus flytrap


Dr. Dominique "Niki" Robertson - Professor

Virus-induced gene silencing, geminivirus resistance



Research Interests

My lab is interested in virus-induced gene silencing and geminivirus-host interactions. Long-term goals are to understand how geminiviruses, which are DNA viruses that transcribe their genes in plant nuclei, interact with plant gene silencing pathways. We are currently mapping an Arabidopsis locus that confers resistance to two different genera of the Geminiviridae, begomoviruses and curtoviruses.


Selected Publications (out of 54)

Hanley-Bowdoin, L., Bejarano, E.R., Robertson, D., Mansoor, S. (2013) Geminiviruses: Masters at redirecting and reprogramming plant processes. Nature Reviews: Microbiology. 11, 777-788.

Robertson, D. (2013) Modulating plant calcium for better nutrition and stress tolerance. ISRN Botany 2013:1-22. (Invited review) http://dx.doi.org/10.1155/2013/952043

Tuttle, J.R., Haigler, C.H., Robertson, D. (2012) Method: Low-cost delivery of the Cotton leaf crumple virus-induced gene silencing system. Plant Methods 8:27-35.

Tuttle. J.R., Idris, A.M., Brown, J.K., Haigler, C.H., Robertson, D. (2008) Geminivirus-mediated Gene Silencing from Cotton Leaf Crumple Virus Is Enhanced by Low Temperature in Gossypium hirsutum. Plant Physiol 148: 41-50.

Jordan, C.V., Shen, W., Hanley-Bowdoin, L., and Robertson, D. (2007) Geminivirus-induced gene silencing of the tobacco retinoblastoma-related gene results in cell death and altered development. Plant Molecular Biology. 65: 163-175.

Smith, C.A., Woloshuk, C.P., Robertson, D., and Payne, G.S. (2007) Silencing of the Aflatoxin Gene Cluster in a Diploid Strain of Aspergillus flavus is Suppressed by Ectopic aflR Expression. Genetics. 176:2077-2086.

Blevins, T., Rajeswaran, R., Shivaprasad, P.V., Beknazariants, D., Si-Ammour, A., Park, H.-S. Vazquez, F. Robertson, D., Meins, F., Hohn, T. and Pooggin, P., 2006 Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing. Nucleic Acids Research 34:6233-6246.

Muangsan, N., Beclin, C., Vaucheret, H., Robertson, D. (2004) Geminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants. Plant J. 38: 1004-1014.

Robertson, D. (2004) VIGS vectors for gene silencing, many targets, many tools. Ann. Rev. Plant Biol. 55: 495-519.

Nagar, S., Hanley-Bowdoin, L., and Robertson, D. (2002) Host DNA Synthesis is Induced by Geminivirus Infection of Differentiated Plant Cells. The Plant Cell. 14:2995-3007.

Turnage, M.A., Muangsan, N., Peele, C.G, and Robertson, D. (2002) Geminivirus-based vector for gene silencing in Arabidopsis. Plant J. 30: 107-114.

Peele, C., Jordan, C.V., Muangsan, N., Turnage, M., Egelkrout, E., Eagle, P., Hanley-Bowdoin, L., and Robertson, D. (2001) Silencing of a meristematic gene using geminivirus-derived vectors. Plant J. 27:357-66.

Bass, H., Nagar, S., Hanley-Bowdoin, L. and Robertson, D. (2000) Chromosome condensation induced by geminivirus infection of mature plant cells. J. Cell Sci. 113: 1149-1160.

Kjemtrup, S., Sampson, K.S., Peele, C., Nguyen, L.V., Conkling, M.A., Thompson, W.F. and Robertson, D. (1998) Gene silencing from plant DNA carried by a geminivirus. The Plant Journal, 14: 91-100.

Nagar, S. Pedersen, T., Carrick, K., Hanley-Bowdoin, L. and Robertson, D. (1995) A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells. The Plant Cell 7: 705-719.