James Petitte

Professor

J_Petitte@ncsu.edu
 

Faculty Memberships:

Educational Background:

  • A.B. (1979) Susquehanna University, Pennsylvania
  • M.S. (1981) University of Maine, Orono, Maine
  • Ph.D (1986) University of Guelph, Guelph, Ontario

Research Interests:

Chickens, turkeys, and ducks supply about 25-30% of the animal protein consumed in the world. This statistic is the result of an 80-year transformation of the poultry industry from backyard flocks to a highly sophisticated, efficient, and economically important industry organized on an international scale. Much of the growth and development of the poultry industry can be attributed to the application of the principles of contemporary biology to genetic selection programs, to the immunology of disease control, and to nutrition programs needed to maintain high levels of production.

The major emphasis of the current research program is on the development of a novel method of gene transfer for the chicken and the application of avian biotechnology to biomedicine and agriculture. Recent work on the production of germline chimeras suggests that the use of cells from the early blastoderm as well as isolated primordial germ cells could be used to insert DNA into the avian germline. The culture of avian embryonic stem cells and primordial germ cells is now under investigation using several approaches. In addition, investigations on the origin of primordial germ cells in the developing embryo offer additional clues to the development of transgenic poultry.


Publications (Selected):

  • Mozdziak, P.E., B. Wysocky, J. Angerman-Stewart, S.L. Pardue, and J.N. Petitte 2006. Production of chick germline chimeras from FACS sorted embryonic gonocytes. Poultry Science, in press.
  • Mozdziak, P.E., Q. Wu, J.M. Bradford, S. L. Pardue, C. Giamario, S. Borwornpinyo, and J. N. Petitte. 2006. Identification of lacZ insertion site and beta-Galactosidase expression in transgenic chickens. Cell and Tissue Research 324: (1) 41 - 53 .(Full Text PDF)
  • Borwornpinyo, S., J. Brake, P. E. Mozdziak, and J. N. Petitte. 2005. Culture of chicken embryos in surrogate eggshells. Poultry Science 84:1477-1482. (Full Text PDF)
  • Mozdziak, P. E., J. Angerman-Stewart, B. Rushton, S. L. Pardue, and J. N. Petitte. 2005. Isolation of chicken primordial germ cells using fluorescence-activated cell sorting. Poultry Science. 84:594-600. (Full Text PDF)
  • Song, Y., S. D�Costa, S. L. Pardue, and J.N. Petitte. 2005. Production of germline chimeric chickens following the administration of a busulfan emulsion. Molecular Reproduction and Development. 70:438-444. (Full Text PDF)
  • Petitte, J.N., G. Liu, and Z. Yang, 2004. Avian pluripotent stem cells. Mechanisms of Development 121 (9): 1159-1168. (Full Text PDF)
  • Petitte, J.N., 2004. Isolation and maintenance of avian ES Cells. Chapter 44, pp. 471-478 in: Hanbook of Stem Cells, Vol. 1. Embryonic Stem Cells. R. Lanza, J. Gearhart, B. Hogan, D. Melton, R. Pedersen, J. Thomson and M. West, eds. Elsivier Academic Press, Burlington, MA
  • Mozdziak, P.E. and J. N. Petitte, 2004. Status of transgenic chicken models for developmental biology. Developmental Dynamics 229(3): 414-421. (Full Text PDF)
  • Mozdziak, P.E., S. Pophal, S. Borwornpinyo,, and J.N. Petitte. 2003. Transgenic chickens expressing �-galactosidase hydrolyze lactose in the intestine. Journal of Nutrition. 133: 3076-3079. (Full Text PDF)
  • Mozdziak, P.E., S. Borwornpinyo, D.W. McCoy, J.N. Petitte. 2003. Development of transgenic chickens expressing bacterial beta-galactosidase. Developmental Dynamics. 226: 439-445.(Full Text PDF)
  • Petitte, J.N. 2003. Strategies for the production of transgenic poultry. Chapter 33, pp. 665-684 in: Poultry Genetics, Breeding and Biotechnology. W.M. Muir and S.E. Aggrey, eds. CABI Publishing.
  • Giamario, C., J.N. Petitte, P.E. Mozdziak. 2003. Hatchability of chicken embryos following somite manipulation. BioTechniques 34:1128-1130. (Full Text PDF)
  • Nolan, C.M., J.K. Killian, J.N. Petitte, R.L. Jirtle, 2001. Imprint status of M6P/IGF2R and IGF2 in chickens. Development Genes Evolution. 211(4):179-83. (PubMed Abstract)
  • D'Costa S, M.J. Kulik , J.N.Petitte, 2000. Expression and purification of biologically active recombinant quail stem cell factor in E. coli. Cell Biol International 24(5):311-317. (PubMed Abstract)
  • Karagenc, L., and J.N. Petitte 2000. Soluble factors and the emergence of chick primordial germ cells in vitro. Poultry Science 79(1):80-85 . (PubMed Abstract)
  • D'Costa, S. and J. N. Petitte, 1999. Characterization of stage-specific embryonic antigen-1 (SSEA-1) expression during early development of the turkey embryo. International J. Dev. Bio. 43:349-356. (PubMed Abstract)
  • Petitte, J.N. and G. Davis, 1999. Breeding and Genetics. Chapter 11, In: The Ostrich: Biology, Production and Health, ed. D.C. Deeming, Wallingford, UK, CAB International. (Full Text PDF)
  • Petitte, J. N., S. D'Costa, L. Karagenc, 1999. Understanding the Origin of Avian Primordial Germ Cells: Implications for Germ Cell Culture and Transgenesis in Poultry. In Transgenic Animals in Agriculture, eds. J.D. Murray, G.B. Anderson, A.M. Oberbauer, and M. M. McGloughlin. CAB International.
  • D�Costa, S. and J.N. Petitte, 1998. Rapid sex determination of turkeys using multiplex PCR. Poultry Science, 77:718-721.
  • Petitte, J.N., L. Karagenc, M. Ginsburg, 1997. The origin of avian primordial germ cells and transgenesis in birds. Poultry Science 76:1084-1092. (PubMed Abstract)
  • Brake, J.T., T.J. Walsh, C.E. Benton, Jr., J.N. Petitte, R. Meijerhof, and G. Penalva. 1997. Egg handing and storage. Poultry Science 76:144-151.
  • Petitte, J.N. and L. Karagenc, 1996. Growth factors during early events in avian embryo development. Poultry and Avian Biology Reviews. 7:75-87.
  • Karagen�, L., Y. Cinnamon, M. Ginsburg, and J. N. Petitte, 1996. The origin of avian primordial germ cells in the pre-streak embryo. Devel. Genet. 19: 290-301. (PubMed Abstract)
  • Petitte, J.N. and M.J. Kulik, 1996. Cloning and characterization of cDNAs encoding two forms of avian stem cell factor. Biochemi. Biophys. Acta. 1307:149-151. (PubMed Abstract)
  • Petitte, J.N. and A.E. Kegelmeyer, 1995. Rapid sex determination of chick embryos using the polymerase chain reaction. Animal Biotechnology, 6: 119-130.
  • Yang, Z. and J.N. Petitte, 1994. Use of avian cytokines in mammalian embryonic stem cell culture. Poultry Science 73:965-974. (PubMed Abstract)
  • Petitte, J.N., A. Elizabeth Kegelmeyer, and Michael J. Kulik, 1994. Isolation of genomic DNA from avian whole blood. BioTechniques 17:664-666.
  • Watt, J.M., J.N. Petitte, and R.J. Etches, 1993. Early Development of the chick embryo. J. Morphology 215:165-182. (PubMed Abstract)
  • Brazolot, C.L., J.N. Petitte, R.J. Etches, and A.M. Verrinder Gibbins, 1991. Efficient Transfection of Chicken Cells by Lipofection, and Introduction of Transfected Blastodermal Cells Into the Embryo. Molecular Reproduction and Development 30:304-312. (PubMed Abstract)
  • Petitte, J.N., M.E. Clark, G., Liu, A.M. Verrinder Gibbins, and R.J. Etches, 1990. Production of Somatic and Germline Chimeras in the Chicken by Transfer of Early Blastodermal Cells. Development 108:185-189. (PubMed Abstract)

Patents:

  • Pardue, S. L., Petitte, J. N., D�Costa, S. and Song, Y. Methods for Gamete Production in Birds. Issued February 17, 2004; US Patent # 6,691,638
  • Petitte, J.N., Ricks, C.A., Spence, S.E. Gene transfer in poultry by introduction of embryo cells in ovo. Issued: February 4, 2003; US Patent #6515199
  • Petitte, J.N., Ricks, C.A., Phelps, P.V., and Williams, C. Gene transfer in chickens by introduction of DNA into muscle in ovo . Issued: May 28, 2002; U.S. Patent # 6395961
  • Pardue, S. L., Petitte, J. N., and D�Costa, S. Methods for Gamete Production in Birds. Issued: March 12, 2002; U.S. Patent #6,354,242
  • Petitte, J. N. and Chang, I. Method of Producing an Undifferentiated Avian Cell Culture using Avian Primordial Germ Cells. Issued: December 25, 2001; U.S. Patent # 6,333,192.
  • Petitte, J. N. and Yang, Z. Veterinary Pharmaceutical Formulation Containing Avian Embryonic Stem cells. Issued: November 3, 1998; U.S. Patent # 5,830,510.
  • Petitte, J. and C. A. Ricks, Apparatus for Injecting Avian Embryo Muscle Tissue In Ovo, Issued: July 28, 1998, U.S. Patent # 5,784,992.
  • Petitte, J. N. and Yang, Z. Avian Embryonic Stem Cells. Issued: August 12, 1997; U.S. Patent # 5,656,479.
  • Petitte, J. N. and Yang, Z. Method of Producing an Avian Embryonic Stem Cell Culture and the Avian Embryonic stem Cell culture Produced by the Process. Issued: August 23, 1994; U.S. Patent # 5,340,740.
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