Robertson Lab

Department of Plant Biology, North Carolina State University






Arabidopsis VIGS

Cotton VIGS



Selected Publications (out of 54)

Hanley-Bowdoin, L., Bejarano, E.R., Robertson, D., Mansoor, S. (2013) Geminiviruses: masters at redirecting and reprogramming plant processes. Nature Reviews: Microbiology 11: 777-788.

Robertson, D. (2013) Modulating plant calcium for better nutrition and stress tolerance. ISRN Botany 2013:1-22.

Villanueva-Alonzo, H.J., López-Ochoa, L., Robertson, D., Guerra-Peraza, O., Minero-Garcia, Y., and Moreno-Valenzuela, O.A. (2013) A new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan Peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. Anaheim. Biotechnology Letters. DOI 10.1007/s10529-013-1146-1

Tuttle, J.R., Haigler, C.H. and Robertson, D. (2012) Method: Low cost delivery of the Cotton leaf crumple virus-induced gene silencing system. Plant Methods 8: 27-35.

Tsou, P.-L., Lee, S.-Y., Allen, N., Winter-Sederoff, H., Robertson, D. (2012) An ER-targeted calcium-binding peptide confers salt and drought tolerance mediated by CIPK6 in Arabidopsis. Planta 235: 539-552.

Idris, A.M., Tuttle, J.R., Robertson, D., Haigler, C.H., Brown, J.K. (2010) Differential Cotton leaf crumple virus-VIGS-mediated gene silencing and viral genome localization in different Gossypium hirsutum genetic backgrounds. Physiol. Mol. Plant Path. 75:13-22.

Bernacki, S., Karimi, M., Hilson, P., Robertson, D. (2010) Virus-Induced Gene Silencing as a Reverse Genetics Tool to Study Gene Function. In:  Methods in Molecular Biology: Plant Developmental Biology. Eds: Lars Hennig Totowa, NJ: Humana.

Tuttle. J.R., Idris, A.M., Brown, J.K., Haigler, C.H., Robertson, D. (2008) Geminivirus-mediated Gene Silencing from Cotton Leaf Crumple Virus Is Enhanced by Low Temperature in Gossypium hirsutum. Plant Physiol 148: 41-50.

Smith, C.A., Robertson, D., Yates, B., Nielsen, D.M., Brown, D., Dean, R.A., Payne, G.A. (2008) The effect of temperature on Natural Antisense Transcript (NAT) expression in Aspergillus flavus. Curr Genet. 54:241-69.

Bernacki, S., Tuttle, J.R., Muangsan, N., and Robertson, D. (2008) Extending functional genomics: VIGS for model and crop plants. In: The Handbook of Functional Genomics. Ed: Kahl, G. and Meksem, K. Series: Plant Genomics, Volume 2. Wiley-VCH

Jordan, C.V., Shen, W., Hanley-Bowdoin, L., and Robertson, D. (2007) Geminivirus-induced gene silencing of the tobacco retinoblastoma-related gene results in cell death and altered development. Plant Molecular Biology. 65:163-175.

Smith, C.A., Woloshuk, C.P., Robertson, D., and Payne, G.S. (2007) Silencing of the Aflatoxin Gene Cluster in a Diploid Strain of Aspergillus flavus is Suppressed by Ectopic aflR Expression. Genetics. 176:2077-2086.

Blevins, T., Rajeswaran, R., Shivaprasad, P.V., Beknazariants, D.,  Si-Ammour, A.,  Park, H.-S. Vazquez, F. Robertson, D., Meins, F., Hohn, T. and Pooggin, P., 2006 Four plant Dicers mediate viral small RNA biogenesis and DNA virus induced silencing. Nucleic Acids Research 34:6233-6246.

Muangsan, N., Beclin, C., Turnage, M., Vaucheret, H., Robertson, D. (2004) Geminivirus VIGS of endogenous genes requires SGS2/SDE1 and SGS3 and defines a new branch in the genetic pathway for silencing in plants. Plant J. 38: 1004-1014.

Hanley-Bowdoin L, Settlage SB, Robertson D (2004) Reprogramming plant gene expression – a prerequisite to geminivirus DNA replication. Mol. Plant Path. 5: 149-156.

Robertson, D. (2004) VIGS vectors for gene silencing, many targets, many tools. Ann. Rev. Plant Biol. 55: 495-519.

Muangsan, N., Robertson, D. (2004) Geminivirus vectors for transient gene silencing in plants. Methods Mol. Biol. 265: 101-116.

Nagar, S., Hanley-Bowdoin, L., and Robertson, D. (2002) Host DNA Synthesis is Induced by Geminivirus Infection of Differentiated Plant Cells. The Plant Cell. 14: 2995-3007.

Egelkrout, E., Mariconti, L., Cella, R., Robertson, D., and Hanley-Bowdoin, L. (2002) Two E2F elements regulate the proliferating cell nuclear antigen promoter during plant development. The Plant Cell. 14: 3225-3236.

Wyatt, S.E., Rashotte, A.M., Shipp, M., Robertson, D. and Muday, G. K. (2002) Mutations in the GPS loci in Arabidopsis disrupt the perception and/or signal transduction of gravitropic stimuli. Plant Physiol. 130:1426-1435.

Turnage, M.A., Muangsan, N., Peele, C.G, and Robertson, D. (2002) Geminivirus-based vector for gene silencing in Arabidopsis. Plant J. 30: 107-114.

Wyatt, S.E., Tsou, P.-L. and Robertson, D. (2002) Expression of the high capacity calcium-binding domain of calreticulin increases bioavailable calcium stores in plants. Transgenic Res. 11: 1-10.

Peele, C., Jordan, C.V., Muangsan, N., Turnage, M., Egelkrout, E., Eagle, P., Hanley-Bowdoin, L., and Robertson, D. (2001) Silencing of a meristematic gene using geminivirus-derived vectors. Plant J. 27: 357-66

Persson, S., Wyatt, S.E., Love, J., Thompson, W.F., Robertson, D., Boss, W.F. (2001) The Ca2+ status of the endoplasmic reticulum is altered by induction of calreticulin expression in transgenic plants. Plant Physiol. 126: 1092-104

Egelkrout, E.M., Robertson, D., and Hanley-Bowdoin, L. (2001) PCNA transcription is repressed through an E2F consensus element and activated by geminivirus infection in mature leaves. Plant Cell, 13: 1437-1452

Staman, K., Blum, U., Louws, F., Robertson, D. (2001) Can simultaneous inhibition of seedling growth and stimulation of rhizosphere bacterial populations provide evidence for phytotoxin transfer from plant residues in the bulk soil to the rhizosphere of sensitive species? J. Chem. Ecol. 27: 807-829

Hanley-Bowdoin, L., Settlage, S.B., Orozco, B.M., Nagar, S. and Robertson, D. (2000) Geminiviruses – Models for plant DNA replication, transcription and cell cycle regulation.  Crit. Rev. Biochem. Mol. Biol. 35: 105-140.

Kong, L.J., Orozco, B.M., Roe, J.L., Nagar, S., Ou, S., Feiler, H.S., Durfee, T., Miller, A.B., Gruissem, W. Robertson, D., and Hanley-Bowdoin, L. (2000) A geminivirus replication protein interacts with the retinoblastoma protein through a novel domain to determine symptoms and tissue specificity of infection in plants. EMBO J.  19: 3485-3495.

Bass, H., Nagar, S., Hanley-Bowdoin, L. and Robertson, D. (2000) Chromosome condensation induced by geminivirus infection of mature plant cells. J. Cell Sci. 113: 1149-1160. (cover photo)

Cox, K, Robertson, D., Fites, R.C. (1999) Characterization of a bifunctional thymidylate synthase, dihydrofolate reductase gene from maize. Plant Mol. Biol. 41: 733-739.

Ullah, M.H., Robertson, D., and Fites, R.C. (1999) A gene for thymidine kinase in plants. (Accession No. AF066050)( PGR 99-048). Plant Physiol. 119:1567.

Scott A, Wyatt S, Tsou PL, Robertson D, Allen NS (1999) Model system for plant cell biology: GFP imaging in living onion epidermal cells. Biotechniques 26: 1128-1132

Kjemtrup, S., Sampson, K.S., Peele, C., Nguyen, L.V., Conkling, M.A., Thompson, W.F. and Robertson, D. (1998) Gene silencing from plant DNA carried by a geminivirus. Plant J, 14: 91-100.

Nagar, S. Pedersen, T., Carrick, K., Hanley-Bowdoin, L. and Robertson, D. (1995) A geminivirus induces expression of a host DNA synthesis protein in terminally differentiated plant cells. The Plant Cell 7: 705-719. (cover photo)


Carson, S., and Robertson, D. (2005) Manipulation and Expression of Recombinant DNA: Core Techniques.  Second edition. Elsevier, Amsterdam

Hardin, C., Pinczes, J., Riell, A., Presutti, D., Miller, M., and Robertson, D. (2001) Cloning, Gene Expression and Protein Purification. Oxford University Press, NY